pdl1 fc chimera protein (R&D Systems)

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R&D Systems pdl1 fc chimera protein

a , Representative (n = 6) 20x CellDIVE immunofluorescence image from days 6-8. GFP = NINJA and Nur77, pink = <t>PDL1,</t> blue = EpCAM, cyan = CD45, white = EpCAM. b , 48 hour in-vitro stimulation with CD3, CD28 and either PDL1 or an Fc control. Representative histogram expression of Perforin, Granzyme A, and Granzyme B with quantification of the percent expression. c , Ex-vivo GP33 peptide stimulation. Representative IFNγ and TNFα flow plots, pre-gated on CD8+ T cells. d , Quantification of the percent of antigen-specific T cells (GP33 and GP34 tetramer-positive CD8+ T cells) that make IFNγ following GP33 stimulation. e , KEGG IFNγ response module enrichment score. f , CD274 transcript expression. g , UMAP reduction with LGR5 expression and PDL1 expression from healthy control and inflammatory colitis conditions. h , representative histogram of PDL1 expression following a 24-hour in-vitro stimulation of primary human β cells and human small intestine organoids with various doses of IFNγ. I , Quantification of the %PDL1+ human β cells and human small intestine organoids following the IFN γ stimulation. J , Human CD274 promoter methylation across various organs and cell types and mouse CD274 promoter methylation in islets and colonic epithelial cells, three mice each, pooled with two technical replicates. To assess statistical significance of the PDL1 stimulations, a one-way ANOVA with Tukey’s multiple comparison test was performed. To assess the statistical significance of the KEGG IFNγ signature, Wilcoxon sign-rank tests were performed. To determine the statistical significance of the in-vitro IFNγ stimulations, a Mixed-effects model (REML) with Šídák’s multiple comparisons test was used. For all statistical tests,*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Pdl1 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+b7/Recombinant+Mouse+PD-L1%2FB7-H1+Fc+Chimera+Protein%2C+CF/bio_rxiv__2025__10__19__683142-342-30-34
Average 93 stars, based on 31 article reviews

pdl1 fc chimera protein - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "CD8 T cells mediate immunosurveillance for neoantigen+ epithelial stem cells in the colon"

Article Title: CD8 T cells mediate immunosurveillance for neoantigen+ epithelial stem cells in the colon

Journal: bioRxiv

doi: 10.1101/2025.10.19.683142

a , Representative (n = 6) 20x CellDIVE immunofluorescence image from days 6-8. GFP = NINJA and Nur77, pink = PDL1, blue = EpCAM, cyan = CD45, white = EpCAM. b , 48 hour in-vitro stimulation with CD3, CD28 and either PDL1 or an Fc control. Representative histogram expression of Perforin, Granzyme A, and Granzyme B with quantification of the percent expression. c , Ex-vivo GP33 peptide stimulation. Representative IFNγ and TNFα flow plots, pre-gated on CD8+ T cells. d , Quantification of the percent of antigen-specific T cells (GP33 and GP34 tetramer-positive CD8+ T cells) that make IFNγ following GP33 stimulation. e , KEGG IFNγ response module enrichment score. f , CD274 transcript expression. g , UMAP reduction with LGR5 expression and PDL1 expression from healthy control and inflammatory colitis conditions. h , representative histogram of PDL1 expression following a 24-hour in-vitro stimulation of primary human β cells and human small intestine organoids with various doses of IFNγ. I , Quantification of the %PDL1+ human β cells and human small intestine organoids following the IFN γ stimulation. J , Human CD274 promoter methylation across various organs and cell types and mouse CD274 promoter methylation in islets and colonic epithelial cells, three mice each, pooled with two technical replicates. To assess statistical significance of the PDL1 stimulations, a one-way ANOVA with Tukey’s multiple comparison test was performed. To assess the statistical significance of the KEGG IFNγ signature, Wilcoxon sign-rank tests were performed. To determine the statistical significance of the in-vitro IFNγ stimulations, a Mixed-effects model (REML) with Šídák’s multiple comparisons test was used. For all statistical tests,*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Figure Legend Snippet: a , Representative (n = 6) 20x CellDIVE immunofluorescence image from days 6-8. GFP = NINJA and Nur77, pink = PDL1, blue = EpCAM, cyan = CD45, white = EpCAM. b , 48 hour in-vitro stimulation with CD3, CD28 and either PDL1 or an Fc control. Representative histogram expression of Perforin, Granzyme A, and Granzyme B with quantification of the percent expression. c , Ex-vivo GP33 peptide stimulation. Representative IFNγ and TNFα flow plots, pre-gated on CD8+ T cells. d , Quantification of the percent of antigen-specific T cells (GP33 and GP34 tetramer-positive CD8+ T cells) that make IFNγ following GP33 stimulation. e , KEGG IFNγ response module enrichment score. f , CD274 transcript expression. g , UMAP reduction with LGR5 expression and PDL1 expression from healthy control and inflammatory colitis conditions. h , representative histogram of PDL1 expression following a 24-hour in-vitro stimulation of primary human β cells and human small intestine organoids with various doses of IFNγ. I , Quantification of the %PDL1+ human β cells and human small intestine organoids following the IFN γ stimulation. J , Human CD274 promoter methylation across various organs and cell types and mouse CD274 promoter methylation in islets and colonic epithelial cells, three mice each, pooled with two technical replicates. To assess statistical significance of the PDL1 stimulations, a one-way ANOVA with Tukey’s multiple comparison test was performed. To assess the statistical significance of the KEGG IFNγ signature, Wilcoxon sign-rank tests were performed. To determine the statistical significance of the in-vitro IFNγ stimulations, a Mixed-effects model (REML) with Šídák’s multiple comparisons test was used. For all statistical tests,*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Techniques Used: Immunofluorescence, In Vitro, Control, Expressing, Ex Vivo, Methylation, Comparison

a , Zoomed out image of Fig. 5a , localization of PDL1, F4/80, and CD11c near NINJA+ epithelium. 20x CellDIVE immunofluorescence image. Green = NINJA and Nur77, white = CD8b, red = F4/80.1, pink = PDL1, yellow = CD11c, orange = EpCAM. b , representative 20x CellDIVE immunofluorescence image of T cell-infiltrated NINJA+ islets. Green = NINJA, white = CD3, pink = PDL1, orange = insulin, blue = DAPI. n = 15, accross 2 experiments c , Violin plot of PDL1 transcript expression in various C57BL/6 mouse small intestine epithelial cell populations.

Figure Legend Snippet: a , Zoomed out image of Fig. 5a , localization of PDL1, F4/80, and CD11c near NINJA+ epithelium. 20x CellDIVE immunofluorescence image. Green = NINJA and Nur77, white = CD8b, red = F4/80.1, pink = PDL1, yellow = CD11c, orange = EpCAM. b , representative 20x CellDIVE immunofluorescence image of T cell-infiltrated NINJA+ islets. Green = NINJA, white = CD3, pink = PDL1, orange = insulin, blue = DAPI. n = 15, accross 2 experiments c , Violin plot of PDL1 transcript expression in various C57BL/6 mouse small intestine epithelial cell populations.

Techniques Used: Immunofluorescence, Expressing

a ,Bubble plot of PDL1 methylation in various tissues and locations. b , bar graph depicting non-CpG CD274 methylation and downstream CpG promoter methylation.

Figure Legend Snippet: a ,Bubble plot of PDL1 methylation in various tissues and locations. b , bar graph depicting non-CpG CD274 methylation and downstream CpG promoter methylation.

Techniques Used: Methylation

Recombinant:

Article Title: Cytotoxic-T-Lymphocyte Antigen 4 Receptor Signaling for Lymphocyte Adhesion Is Mediated by C3G and Rap1
Article Snippet: Anti-Hck (N-30), anti-CD28 (PV-1), anti-Epac (N-16), anti-Fgr (M-60), anti-Erk1 (K-23), anti-Lck (3A5), anti-Zap70 (4H386), and anti-CTLA-4 (9094) were obtained from Santa Cruz Biotechnology. .. Recombinant mouse B7-1/CD80 Fc chimera was purchased from R&D Systems. .. A mouse interleukin 2 enzyme-linked immunosorbent assay (ELISA) kit was purchased from Aviva System Biology.

Article Title: Activity of murine surrogate antibodies for durvalumab and tremelimumab lacking effector function and the ability to deplete regulatory T cells in mouse models of cancer
Article Snippet: .. Recombinant mouse PD-1 Fc chimera protein (R&D Systems, catalog #1021-PE-100), recombinant mouse B7-1/CD80 Fc chimera His-tag protein (R&D Systems, catalog #740-B1-100), and purified anti-mouse PD-L1 clone 10 F.9G2 (BioLegend, catalog #124302) were conjugated with DyLight-650 (Innova Biosciences) according to the manufacturer’s directions. .. PD-L1 Fc was conjugated with europium cryptate trisbipyridine–N-hydroxysuccinimide (Cisbio) according to the manufacturer’s directions, and the cryptate conjugate assay dilution factor was determined by comparing the fluorescence (at 590 nm) with the supplied calibrator.

Article Title: Methods and materials for producing T cells
Article Snippet: Lymph node CD8+ T cells (OT-1 PL Rag−/−) obtained from TCR transgenic mice were stimulated in vitro with an antigen-presenting construct in the presence of IL-12 (2 ng/mL; Peprotech, Inc.) for 48 to 72 hours. .. The antigen-presenting construct included sulfate latex microspheres (Life Technologies, Cat. No. 537227) immobilized with DimerX I (a recombinant dimeric mouse H-2Kb:Ig fusion protein obtained from BD Biosciences; Cat. No. 550750) and a recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B1). .. The antigen-presenting construct included sulfate latex microspheres (Life Technologies, Cat. No. 537227) immobilized with DimerX I (a recombinant dimeric mouse H-2K^b:Ig fusion protein obtained from BD Biosciences; Cat. No. 550750) and a recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B1).

Article Title: Methods and materials for producing T cells
Article Snippet: Lymph node CD8+ T cells (OT-1 PL Rag−/−) obtained from TCR transgenic mice were stimulated in vitro with an antigen-presenting construct in the presence of IL-12 (2 ng/mL; Peprotech, Inc.) for 48 to 72 hours. .. The antigen-presenting construct included sulfate latex microspheres (Life Technologies, Cat. No. S37227) immobilized with DimerX I (a recombinant dimeric mouse H-2Kb:Ig fusion protein obtained from BD Biosciences; Cat. No. 550750) and a recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B1). .. The antigen-presenting construct included sulfate latex microspheres (Life Technologies, Cat. No. S37227) immobilized with DimerX I (a recombinant dimeric mouse H-2K^b:Ig fusion protein obtained from BD Biosciences; Cat. No. 550750) and a recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B1).

Article Title: Materials and methods for increasing immune responses
Article Snippet: .. The antigen constitutes sulfate latex microspheres (Life Technologies Cat. No. S37227) bearing DimerX Ig-recombinant dimeric mouse H-2Kb: Ig fusion protein (BD Biosciences, Cat. No. 550750) with recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B). ..

Article Title: IL-21 promotes differentiation of naive CD8 T cells to a unique effector phenotype.
Article Snippet: .. B7-1 in the form of a recombinant mouse B7-1/Fc chimera (R&D Systems) was coimmobilized using 0.15 g/107 microspheres. ..

Purification:

Construct:

Article Title: Methods and materials for producing T cells
Article Snippet: Lymph node CD8+ T cells (OT-1 PL Rag−/−) obtained from TCR transgenic mice were stimulated in vitro with an antigen-presenting construct in the presence of IL-12 (2 ng/mL; Peprotech, Inc.) for 48 to 72 hours. .. The antigen-presenting construct included sulfate latex microspheres (Life Technologies, Cat. No. S37227) immobilized with DimerX I (a recombinant dimeric mouse H-2Kb:Ig fusion protein obtained from BD Biosciences; Cat. No. 550750) and a recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B1). .. The antigen-presenting construct included sulfate latex microspheres (Life Technologies, Cat. No. S37227) immobilized with DimerX I (a recombinant dimeric mouse H-2K^b:Ig fusion protein obtained from BD Biosciences; Cat. No. 550750) and a recombinant mouse B7-1/CD80 Fc chimeric protein (R&D Systems, Cat. No. 740-B1).

Review